Molecular mechanism of granulocytic differentiation of human promyelocytic leukemia HL-60 cells induced by all-trans retinoic acid / 药学学报
Acta Pharmaceutica Sinica
;
(12): 22-28, 2004.
Article
in English
| WPRIM
| ID: wpr-301156
ABSTRACT
<p><b>AIM</b>To elucidate the molecular mechanism of granulocytic differentiation of human promyelocytic leukemia HL-60 cells induced by all-trans-retinoic acid (ATRA).</p><p><b>METHODS</b>Flow cytometry was used to determine the cell cycle changes of HL-60 cells upon ATRA treatment. Gene expression profiles of HL-60 cells induced by 1 mumol.L-1 ATRA were obtained by using cDNA microarrays containing 9,984 genes and expressed sequence tags (ESTs).</p><p><b>RESULTS</b>Cell cycle analysis showed that 48%-73% of cells were arrested at G1/G0 phase upon ATRA treatment; cDNA microarray results demonstrated that the expression of genes encoding adhesion molecules, tissue remodeling proteins, transporters and ribosomal proteins were up-regulated in ATRA treated of HL-60 cells. Several genes involved in oxidase activation pathway were also differentially expressed.</p><p><b>CONCLUSION</b>ATRA treatment induced growth arrest and differentiation in HL-60 cells, which is associated with regulation of the oxidase activation pathway and the expression of tissue remodeling proteins.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Tretinoin
/
Cell Cycle
/
Cell Differentiation
/
HL-60 Cells
/
Oligonucleotide Array Sequence Analysis
/
Gene Expression Profiling
/
Granulocytes
/
Antineoplastic Agents
Limits:
Humans
Language:
English
Journal:
Acta Pharmaceutica Sinica
Year:
2004
Type:
Article
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