Suppression of experimental abdominal aortic aneurysm by saturated hydrogen saline: a preliminary study with rats / 中华外科杂志
Chinese Journal of Surgery
;
(12): 437-441, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-301264
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of saturated hydrogen saline on the prevention of abdominal aortic aneurysm (AAA) induced by calcium chloride in a rat model.</p><p><b>METHODS</b>In healthy male Sprague-Dawley rats, AAA was induced by infiltration of abdominal arota with 0.5 mol/L calcium chloride. Saturated hydrogen saline (5 ml·kg(-1)·d(-1)) or saline was administred intraperitoneally once daily. Twenty-eight days later, the diameter of the aorta was measured, and the aortic tissue was exercised for histological examination. Pro-inflammatory cytokines (tumor necrosis factor α (TNF-α), IL-1β) in AAA tissue were detected with ELISA. The protein expression and mRNA expression of matrix metalloproteinase 2 (MMP-2) and MMP-9 in AAA tissue were observed by immunohistochemistry staining and real-time PCR.</p><p><b>RESULT</b>The aorta diameter of the experiment group and control group were (2.2 ± 0.3) mm and (3.4 ± 0.5) mm, the tissue IL-1β levels were (81 ± 29) ng/L and (165 ± 51) ng/L, the tissue TNF-α levels were (109 ± 46) ng/L and (360 ± 51) ng/L, the relative mRNA expressions were 2.4 ± 1.0 and 11.8 ± 2.9, the relative mRNA expressions were 2.9 ± 0.6 and 6.7 ± 1.0 (t = 4.055 to 10.406, P < 0.05). Compared with the control group, the infiltration of inflammation, the injury of elastic fibers in the vessel wall, and the positive expression of MMP-2 and 9 protein of the experiment group were all reduced.</p><p><b>CONCLUSIONS</b>Saturated hydrogen saline prevents the degradation of elastin in vessel wall and ameliorates the formation and development of AAA, which may be associated with its anti-inflammatory effects, thereby reduces the MMP-2 and 9 mRNA and protein expression.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Aorta, Abdominal
/
Pathology
/
Pharmacology
/
Sodium Chloride
/
Tumor Necrosis Factor-alpha
/
Rats, Sprague-Dawley
/
Aortic Aneurysm, Abdominal
/
Matrix Metalloproteinase 2
/
Matrix Metalloproteinase 9
/
Disease Models, Animal
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2013
Type:
Article
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