The effect of Crocin against hypoxia damage of myocardial cell and its mechanism / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 453-457, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-301538
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the protective effect of Crocin against hypoxia damage of cardiac myocytes of neonatal rats and the regulation of HIF-1 and prolyhydroxylase (PHDs).</p><p><b>METHODS</b>A model of CoCl2 simulated hypoxia damage was established in primary cultural myocardial cell. Expression levels of HIF-1alpha, VEGF, iNOS, as well as PHD1, 2, 3 protein in myocardial cells were detected by Western blot.</p><p><b>RESULTS</b>Compared with CoCl2 group, the viability of myocardial cell was significantly increased after treated 24 h at 10(-5)mol/L Crocin (P < 0.01), HIF-1alpha, VEGF and iNOS were expressed higher than those in Crocin + CoCl2 group (P < 0.01), the expression of PHD2 was significantly increased (P < 0.01), while the expression of PHD3 was remarkably reduced in Crocin + CoCl2 Group (P < 0.01).</p><p><b>CONCLUSION</b>Crocin has better protective effect on hypoxic damage of myocardial cell. The mechanisms of protective effect of Crocin may be related to the activation of HIF-1-mediated pathway of the hypoxia response. PHDs may be involved in the pathophysiology regulated process of myocardial cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Carotenoids
/
Cell Hypoxia
/
Cells, Cultured
/
Rats, Sprague-Dawley
/
Procollagen-Proline Dioxygenase
/
Homeodomain Proteins
/
Myocytes, Cardiac
/
Vascular Endothelial Growth Factor A
/
Nitric Oxide Synthase Type II
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2010
Type:
Article
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