Preparation of clenbuterol monoclonal antibody with subtractive immunization method / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 573-576, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-301558
ABSTRACT
<p><b>AIM</b>To obtain Clenbuterol monoclonal antibodies.</p><p><b>METHODS</b>Clenbuterol complete antigen was prepared with diazotization method. BALB/c mice was immunized with subtractive immunization, Clenbuterol monoclonal antibody was prepared with rule hybridoma technique.</p><p><b>RESULTS</b>The mice obtained tolerance to BSA by subtractive immunization. The rate of the hybridoma cell with positive reaction which had obtained was 8.2%, and the specific clenbuterol monoclonal antibody was obtained at last.</p><p><b>CONCLUSION</b>Monoclonal antibodies to micromolecule contaminant be prepared by subtractive immunization, could decrease the workload in the bolting of monoclonal antibodies, and increase the chance to obtain the antibody of expected.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Serum Albumin, Bovine
/
Immunization
/
Clenbuterol
/
Allergy and Immunology
/
Hybridomas
/
Metabolism
/
Methods
/
Mice, Inbred BALB C
/
Antibodies, Monoclonal
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2009
Type:
Article
Similar
MEDLINE
...
LILACS
LIS