The chronic effect of palmitic acid on apoptosis of pancreatic islet beta-cells and the mechanism / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 553-556, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-301563
ABSTRACT
<p><b>AIM</b>To investigate the chronic effect of palmitic acid (PA) on apoptosis of pancreatic islet beta-cells and the possible mechanism.</p><p><b>METHODS</b>Insulinoma cell line (MIN6 cells) were used in this study. After being incubated in PA (0.1 - 1.6 mml/L) for 24 and 48 hours, MTT method was used to evaluate the livability. After being incubated for 48 h, Hoechst-PI and Annexin-V-FTTC/PI FACS were used to estimate the apoptosis in each group, Western-blotting assay was used to estimate the protein level of p-Akt, Akt, Bax and Bcl-2.</p><p><b>RESULTS</b>Chronic PA dose-dependently (1) decreased the availability and increased the apoptosis of MIN6 cells; (2) decreased the phosphorylation of Akt and Bcl-2, but had no significant effects on Akt and Bax.</p><p><b>CONCLUSION</b>Chronic PA dose-dependently induced apoptosis of MIN6 cells, and this effect was possibly regulated by Akt/Bcl-2.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Physiology
/
Signal Transduction
/
Cells, Cultured
/
Islets of Langerhans
/
Proto-Oncogene Proteins
/
Apoptosis
/
Oxidative Stress
/
Palmitic Acid
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2009
Type:
Article
Similar
MEDLINE
...
LILACS
LIS