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γδ T lymphocyte function and the polymorphism of T cell receptor V δ chain in lungs of asthmatic patients / 中国医学科学院学报
Acta Academiae Medicinae Sinicae ; (6): 136-140, 2010.
Article in Chinese | WPRIM | ID: wpr-301579
ABSTRACT
<p><b>OBJECTIVE</b>To observe the function of gamma delta T lymphocytes and the polymorphism of T cell receptor V delta chain in the lungs of asthmatic patients and explore the role of gamma delta T cells in airway inflammation.</p><p><b>METHODS</b>Bronchoalveolar lavage fluid BALF was obtained from 7 asthmatic patients and 7 healthy control individuals. The percentage of gamma delta T cell in BALF was measured by flow cytometry. The gamma delta T cell in BALF was purified by magnetic labeled beads. Proliferous activity was examined by MTT assay. Cytokines secreted by gamma delta T cells in medium was assessed by enzyme-linked immunosorbent assay. Polymorphism of T cell receptor V delta chain was detected by RT-PCR and gene scan analysis.</p><p><b>RESULTS</b>The proportion of gamma delta T cell in the BALF of asthmatic patients [(6.39+/-0.71)%] was significantly higher than that in control subjects [(2.62+/-0.37)%] (P<0.01). The proportion of macrophage in the BALF of asthmatic patients [(81+/-4)] was significantly lower than that in control subjects [(86+/-2)] (P<0.05). The proliferation rate of asthmatic patients [(284.2+/-43.6)%] was significantly higher than that of control subjects [(217.5+/-59.5)%] (P<0.05). Interleukin-4 secreted by gamma delta T cells of asthmatic patients [(18.9+/-3.1) pg/ml)] significantly increased when compared with the control subjects [(14.1+/-3.0) pg/ml] (P<0.05). The polymorphism of T cell receptor V delta chain was not significantly different between these two groups.</p><p><b>CONCLUSIONS</b>The increase of gamma delta T cells in the lung of asthmatic patients further exacerbates Th1/Th2 disturbance and airway inflammation. Antigen recognition by gamma delta T cells is non-specific.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Polymorphism, Genetic / Asthma / Immunoglobulin Variable Region / Bronchoalveolar Lavage Fluid / Case-Control Studies / Cytokines / T-Lymphocyte Subsets / Genes, T-Cell Receptor delta / Genes, T-Cell Receptor gamma / Cell Biology Type of study: Observational study Limits: Adult / Female / Humans / Male Language: Chinese Journal: Acta Academiae Medicinae Sinicae Year: 2010 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Polymorphism, Genetic / Asthma / Immunoglobulin Variable Region / Bronchoalveolar Lavage Fluid / Case-Control Studies / Cytokines / T-Lymphocyte Subsets / Genes, T-Cell Receptor delta / Genes, T-Cell Receptor gamma / Cell Biology Type of study: Observational study Limits: Adult / Female / Humans / Male Language: Chinese Journal: Acta Academiae Medicinae Sinicae Year: 2010 Type: Article