Heat shock induced the expression of major histocompatibility complex class transactivator and human leukocyte antigen-DR in Jurkat cells / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 746-750, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-301615
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of a non-lethal heat shock, in comparison with the treatment of interferon-gamma (IFN gamma), on the expression of major histocompatibility complex transactivator (CTA) and its downstream target gene of the human leukocyte antigens (HLA)-DR in Jurkat cells.</p><p><b>METHODS</b>The changes of CTA mRNA in Jurkat cells before and after the treatment of heat shock or IFN gamma were detected using real time RT-PCR. The changes of CTA protein were detected with Western blot. The expression of HLA-DR was detected with flow cytometry. CTA mRNA and protein were induced in Jurkat cells under heat shock, but not with IFN-gamma. The expression of HLA-DR gene significantly increased after recovery (P<0.01).</p><p><b>CONCLUSION</b>The expressions of CTA and HLA-DR in Jurkat cells remarkably increase after heat shock, indicating that heat shock may help reconstruct relevant genes in cells with immunologic gene deficiencies.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
RNA, Messenger
/
Nuclear Proteins
/
HLA-DR Antigens
/
Trans-Activators
/
Heat-Shock Response
/
Jurkat Cells
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2009
Type:
Article
Similar
MEDLINE
...
LILACS
LIS