Inhibitory role of SirT7 in the growth of P19 cell line / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 724-727, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-301619
ABSTRACT
<p><b>OBJECTIVE</b>To study the regulatory role of SirT7, one of class histone deacetylases, on the proliferation of mouse embryonal carcinoma cell line P19.</p><p><b>METHODS</b>We used an expression plasmid of SirT7 (151-402 amino acid residues) and its vector respectively to establish a stably expressed SirT7 and its control P19 cell lines. Recombinant DNA techniques, Western blot, cell growth curve, and flow cytometry were used in this paper.</p><p><b>RESULTS</b>Compared with the control cells, the P19 cells had significantly lower growth rate in stably expressed SirT7. G1 to S cell cycle arrests were only seen in the SirT7 over-expressed cell line.</p><p><b>CONCLUSION</b>SirT7 play a dominant role in the grow inhibition of the P19 cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Plasmids
/
Transfection
/
Cell Cycle
/
Sirtuins
/
Cell Line, Tumor
/
Cell Proliferation
/
Genetic Vectors
/
Genetics
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2009
Type:
Article
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