Inhibitory role of SirT7 in the growth of P19 cell line / 中国医学科学院学报

ABSTRACT

<p><b>OBJECTIVE</b>To study the regulatory role of SirT7, one of class histone deacetylases, on the proliferation of mouse embryonal carcinoma cell line P19.</p><p><b>METHODS</b>We used an expression plasmid of SirT7 (151-402 amino acid residues) and its vector respectively to establish a stably expressed SirT7 and its control P19 cell lines. Recombinant DNA techniques, Western blot, cell growth curve, and flow cytometry were used in this paper.</p><p><b>RESULTS</b>Compared with the control cells, the P19 cells had significantly lower growth rate in stably expressed SirT7. G1 to S cell cycle arrests were only seen in the SirT7 over-expressed cell line.</p><p><b>CONCLUSION</b>SirT7 play a dominant role in the grow inhibition of the P19 cells.</p>