Role of Ezh2 in the all-trans retinoic acid induced P19 neural differentiation / 中国医学科学院学报

ABSTRACT

<p><b>OBJECTIVE</b>To study the role of Ezh2 in the all-trans retinoic acid RA induced P19 neural differentiation.</p><p><b>METHODS</b>The expression of Ngn1 in the RA induced P19 cells was detected at the mRNA and protein levels using real time RT-PCR and Western blot assays. The binding of Ezh2 and H3K27me3 on the Ngn 1 promoter was analyzed using chromatin immunoprecipitation assay.</p><p><b>RESULT</b>In the RA induced P19 cells, the recruitment of Ezh2 and its methylated substrate H3K27me3 on the promoter of Ngn 1 gene elevated in the first 2 days, and then declined rapidly, followed by the initiation of neuronal differentiation.</p><p><b>CONCLUSIONS</b>Ezh2 produces a repressive histone mark H3K27me3 in the early stage of RA induced P12 cells. By avoiding the premature expression of Ngn1 gene, Ezh2 can ensure the normal differentiation of P19 cells.</p>