Sensitivity of interferon-gamma release assay T-SPOT. TB in diagnosing extrapulmonary tuberculosis / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 449-452, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-301674
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the sensitivity of an interferon-gamma release assay T-SPOT. TB in the diagnosis of bacteriologically or histologically confirmed extrapulmonary tuberculosis.</p><p><b>METHOD</b>Totally 31 patients with bacteriologically or histologically confirmed extrapulmonary tuberculosis in Peking Union Medical College Hospital received T-SPOT. TB assay to detect early secreting antigen target 6 or culture filtrate protein 10 peptides-specific T cells in the peripheral blood mononuclear cells (PBMCs).</p><p><b>RESULTS</b>T-SPOT. TB assay showed positive results in 29 patients with extrapulmonary tuberculosis and the sensitivity was 93.5% (95% CI 84.8% - 100%). The median of spot forming cells (SFCs) in response to early secreting antigen target 6 peptides was 196/10(6) PBMCs (interquartile range, 72-532/10(6) PBMCs), the median of SFCs in response to culture filtrate protein 10 peptides was 276 SFCs/10(6) PBMCs (interquartile range, 72-568/10(6) PBMCs), and the median of the incorporate SFCs was 612/10(6) PBMCs (interquartile range, 192-1 152/10(6) PBMCs).</p><p><b>CONCLUSION</b>T-SPOT. TB is highly sensitive in diagnosing extrapulmonary tuberculosis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tuberculosis
/
Leukocytes, Mononuclear
/
Sensitivity and Specificity
/
Interferon-gamma
/
Diagnosis
/
Interferon-gamma Release Tests
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2009
Type:
Article
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