Levels of T-Lymphocyte Subsets, IL-17, IL-35 and IFN-γ in Peripheral Blood and Their Clinical Significance in Patients with Multiple Myeloma / 中国实验血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore the levels of T-lymphocyte subsets and IL-17, IL-35, IFN-γ in peripheral blood of patients with multiple myeloma(MM) and their clinical significance.</p><p><b>METHODS</b>A total of 86 MM patients in our hospital from January 2014 to January 2017 were enrolled in MM group and 30 healthy persons were enrolled in control group, the CD4/CD8T cells ratio, CD4CD25CD127Treg level in peripheral blood were detected by flow cytometer. The levels of IL-17, IL-35 and IFN-γ in peripheral serum were detected by ELISA, and the differences of detected indicators between different groups were compared.</p><p><b>RESULTS</b>Compared with control group, the proportion of CD4T cells and CD4/CD8T cells ratio decreased, the proportion of CD8T cells and Treg increased in MM group. The differences of T lymphocyte subsets level between group III stage of MM and control group were statistically significant (P<0.05). With enhancing of clinical stages, Treg level showed a increasing trend, especially in III stage (P<0.05), the serum level of IL-17 as followed in turn III stage>II stage>I stage>control, the serum level of IL-35 and IFN-γ as followed in turn control>I stage>II stage>III stage (P<0.05). In terms of disease status, the propurtion of Treg cells as fllowed in turn disease progression stage>stable stage>control (P<0.05), the serum level of IL-17 as followed in turn also disease progression stage>stable stage (P<0.05), while the serum level of IL-35 and IFN-γ as followed in turn control>disease table stage>progression stage (P<0.05).</p><p><b>CONCLUSION</b>The abnomal level of T-lymphocyte subsets, Treg, IL-17, IL-35 and IFN-γ are related with progression and prognosis of MM patients.</p>