Experimental research on spine fusion induced by tissue engineered bone / 中国医学科学院学报

ABSTRACT

<p><b>OBJECTIVE</b>To construct a reasonable substitute for the autograft bone in vitro and transplant it back into the rabbit models to induce the spine fusion.</p><p><b>METHODS</b>The bone marrow stem cell from the seven New Zealand rabbits were cultured. Recombinant human bone morphogenetic protein-4 (rhBMP-4) that has been proved to be bioactive was obtained by the way of genetic engineering. Using the vacuum freezing machine to mix a certain quantity of rhBMP-4 into type I collagen to form a new kind of carrier. Animal model of spine facet process fusion was used. Bone marrow stem cells combined with rhBMP-4 and I type collagen were implanted between the facet process to induce the spine fusion. type I collagen and bone marrow stem cell was used in the controlled group.</p><p><b>RESULTS</b>New bone formation was obvious in the test group. The facet joint was fused very well in this side. No bone formation was present on the other side.</p><p><b>CONCLUSIONS</b>The new composite bone marrow stem cells, rhBMP-4 and type I collagen was an ideal kind of substitute for the autograft bone.</p>