Laminin and its alpha 6 integrin receptor in the regulation of human hepatocellular carcinoma cell phenotypes / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 31-35, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-301913
ABSTRACT
<p><b>OBJECTIVE</b>To study the role of integrin alpha 6 in cell attachment, spread, survival/proliferation and differentiation of human hepatocellular carcinoma (HCC) BEL-7402 cells on various substrates by monoclonal antibody against the extracellular domain of alpha 6 subunit (IA6ED McAb).</p><p><b>METHODS</b>The effect of McAb on attachment and spread of BEL-7402 cells on LN or FN substrate was examined. MTT analysis was used to examine the cell survival/proliferation, gelatin zymography to the matrix metalloproteinases (MMPs) secreted by BEL-7402 cells, and microparticle immunoabsorbent kit to AFP secretion while the cells were cultured on LN-, FN- or Matrigel-coated substrates.</p><p><b>RESULTS</b>The cell attachment, spread and survival/proliferation were inhibited. Moreover importantly, the malignant cell dedifferentiation and abnormal differentiation on LN-coated substrate were also strongly inhibited by IA6ED McAb.</p><p><b>CONCLUSION</b>LN and integrin alpha 6 regulate human HCC cell phenotypes of survival/proliferation and differentiation. The phenotypes of dedifferentiation and abnormal differentiation can be reversed by blocking the interaction between integrin alpha 6 and LN using IA6ED McAb, which may lower metastatic potency of tumor cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Phenotype
/
Physiology
/
Tumor Cells, Cultured
/
Cell Adhesion
/
Laminin
/
Receptors, Laminin
/
Carcinoma, Hepatocellular
/
Integrin alpha6
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2003
Type:
Article
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