Influence of 4-aminopyridine on voltage-activated K(+) current and cell proliferation in small cell lung cancer / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 230-233, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-301967
ABSTRACT
<p><b>OBJECTIVE</b>To study the inhibition of voltage-activated K(+) conductance and cell proliferation by 4-aminopyridine (4-AP) in the human small-cell lung cancer (SCLC).</p><p><b>METHODS</b>Inhibition of voltage-activated K(+) current by 4-AP through the whole-cell patch-clamp technique in SCLC cell line was studied. The influence on the cell-cycle by 4-AP was observed by flow cytometry to identify the in vitro inhibition by 4-AP to the cell proliferation of the SCLC cell line.</p><p><b>RESULTS</b>Exposure of the tumor cells to 5 mmol/L 4-AP reduced the peak outward K(+) current (evoked by a depolarization to +80 mV) from 1.22 +/- 0.11 nA (n = 30) to 0.59 +/- 0.10 nA (n = 28). Flow cytometry results showed that cell population accumulated in the G(0)/G(1) phase and a significantly reduced proportion in the S phase and G(1)/G(2) phase cells after having been exposed to 4-AP for three days. Incubation of the SCLC cells with 0.1, 5, 10, 15, 20 mmol/L 4-AP resulted in a concentration-and time-dependent reduction in the number of viable cells as compared with the control.</p><p><b>CONCLUSION</b>The voltage-activated K(+) channels expressed by SCLC play an important role in SCLC cell proliferation. The proliferation of the SCLC cells is inhibited by K(+) channel antagonists.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Tumor Cells, Cultured
/
4-Aminopyridine
/
Cell Division
/
Carcinoma, Small Cell
/
Potassium Channels, Voltage-Gated
/
Potassium Channel Blockers
/
Lung Neoplasms
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2002
Type:
Article
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