Delayed rectifier K(+) channel regulated by cyclooxygenase-2 in human gastric cancer cell / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 440-443, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-301993
ABSTRACT
<p><b>OBJECTIVE</b>To correlate delayed rectifier K(+) channel to cyclooxygenase-2 (COX-2) in onco genesis of human gastric cancer cell.</p><p><b>METHODS</b>Human COX-2 encoding gene was cloned with RT-PCR strategy and its antisense recombinant eukaryotic expression vector was constructed. COX-2 highly expressed human gastric cancer cell line SGC7901 was stably transfected with the antisense vector. The whole-cell recording technique of perforated patch clamp was employed to observe the change of delayed rectifier K(+) current (I(k)) of SGC7901 after gene transfer or treatment with COX-2 inhibitor indomethacin. MTT was also performed to determine the effect of delayed rectifier K(+) channel inhibitors on cell growth.</p><p><b>RESULTS</b>Stably transfected cell (7901-AS) was obtained and a down-regulated expression of COX-2 protein and mRNA in the cell was achieved. Patch clamp recording showed that both SGC7901 and 7901-AS cells had a typical delayed rectifier K(+) current. However, I(k) was significantly lower (P < 0.01) in transfected cell or cell treated with indomethacin at each test potential. The altered I(k) could be entirely recovered after drug removal from the cells. K(+) channel blockers tetraethylammonium (TEA) and 4-aminopyridine (4-AP) could retard the growth of SGC7901 and the transfected cell in a dose-dependent manner.</p><p><b>CONCLUSION</b>Delayed rectifier K(+) channel, existing in human gastric cancer cell line SGC7901, is related to the growth of the cell. The highly expressed COX-2 may affect the biological behavior of gastric cancer cell by regulating this ion channel.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Stomach Neoplasms
/
Tumor Cells, Cultured
/
Potassium Channels
/
Cell Division
/
Prostaglandin-Endoperoxide Synthases
/
Potassium Channels, Voltage-Gated
/
Cyclooxygenase 2
/
Delayed Rectifier Potassium Channels
/
Isoenzymes
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2002
Type:
Article
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