Differentially expressed gene in nasopharyngeal carcinoma cell lines with various metastatic potentialities / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 430-434, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-301995
ABSTRACT
<p><b>OBJECTIVE</b>To investigate gene expression profile in nasopharyngeaL carcinoma (NPC) cell lines with different metastatic potentialities, in order to identify new candidate genes related to the development, progress and metastasis of NPC.</p><p><b>METHODS</b>The mRNA expressions of high metastatic NPC cell line 5-8F, tumorigenic but nonmetastatic NPC cell line 6-10B and non-tumorigenic NPC cell line 13-9B (3 sublines of SUNE-1) were investigated by cDNA microarray containing 14 000 cDNA clones. The alterations in gene expression levels were confirmed by reverse-transcription PCR.</p><p><b>RESULTS</b>There were 82 differentially expressed genes comparing 5-8F and 13-9B; 38 differentially expressed genes comparing 6-10B and 13-9B; 54 comparing 5-8F and 6-10B. There were 12 common differentially expressed genes comparing 6-10B, 5-8F and 13-9B; 14 common differentially expressed genes comparing 5-8F and 13-9B, 6-10B. The expressions of the above genes were involved in metabolism, transcription, differentiation, apoptosis and signal transduction.</p><p><b>CONCLUSION</b>The gene expression profile in nasopharyngeal carcinoma cell lines is an important index in the search of new candidate genes related to NPC.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Tumor Cells, Cultured
/
Gene Expression Regulation, Neoplastic
/
Cell Line
/
Nasopharyngeal Neoplasms
/
DNA, Complementary
/
Oligonucleotide Array Sequence Analysis
/
Gene Expression Profiling
/
Genetics
/
Neoplasm Metastasis
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2002
Type:
Article
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