Relationship between hepatitis B virus genotype, BCP/Pre-C region mutations and risk of hepatocellular carcinoma in Guangxi Zhuang Autonomous Region / 中华流行病学杂志
Chinese Journal of Epidemiology
;
(12): 725-729, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-302094
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the relationship between hepatitis B virus (HBV) genotype, the mutation in basic core promoter (BCP) region/pre-core (Pre-C) region and the incidence of hepatocellular carcinoma (HCC) in Fusui county of Guangxi Zhuang Autonomous Region (Guangxi), a area with high incidence of HCC.</p><p><b>METHODS</b>In this case-control study, 53 HCC patients and 70 asymptomatic HBV carriers were enrolled. Blood samples were collected from them for serum separation and HBV DNA extraction. The DNA sequences of the S region and BCP/Pre-C region of HBV was determined by direct sequencing following nested-PCR amplification. The relationship between the genotype, gene mutation of HBV and the incidence of HCC was analyzed.</p><p><b>RESULTS</b>The mutation rates of the A1762T/G1764A in the BCP region and the T1858C in the Pre-C region of HBV were significantly higher in HCC group than in control group (94.3% vs. 75.7%, P = 0.006; 50.9% vs. 31.4%, P = 0.029). The mutation rate of A1775G was significantly higher in control group (28.6%) than in HCC group (13.2%) (P = 0.041). Multiple logistic regression analysis indicated that A1762T/G1764A and T1858C mutations are the risk factors for the development of HCC (OR = 5.459, 95% CI 1.397-21.332, P = 0.015; OR = 3.881, 95% CI 1.462-10.305, P = 0.006). A1775G is the protective factor in the development of HCC (OR = 0.192, 95% CI 0.059-0.622, P = 0.006).</p><p><b>CONCLUSION</b>The present investigation showed that BCP A1762T/G1764A, A1775G and Pre-C T1858C mutations are correlated with the incidence of HCC in Fusui county of Guangxi.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
DNA, Viral
/
Case-Control Studies
/
China
/
Hepatitis B virus
/
Polymerase Chain Reaction
/
Epidemiology
/
Incidence
/
Risk Factors
/
Sequence Analysis, DNA
Type of study:
Etiology study
/
Incidence study
/
Observational study
/
Prognostic study
/
Risk factors
Limits:
Humans
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Epidemiology
Year:
2015
Type:
Article
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