Influence of high mobility group box 1 on migration of human cord blood CD34(+) cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 422-425, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-302119
ABSTRACT
The objective of study was to explore the influence of high mobility group box 1 (HMGB1) on migration of cord blood CD34(+) cells and their mechanism of migration. The expressions of receptor for advanced glycation end products (RAGE), toll-like receptor-2 (TLR2) and TLR4 were detected by flow cytometry. The CD34(+) cells in umbilical cord blood (CB) were enriched by MiniMACS and were exposed to various concentration of HMGB1 (10, 50, 100, 1, 000 ng/ml), then the migration effect of HMGB1 on umbilical cord blood (UCB) CD34(+) cell count was determined by microscopy, the chemotactic index was calculated. The CD34(+) cells untreated with HMGB1 were used as control. The results indicated that the purity of the isolated CD34(+) cells was more than 98%. The HMGB1 could promote the migration of CD34(+) cells, and the migration effect of HMGB1 on CD34(+) cells in certain concentrations gradually increased along with raise of concentration, the strongest effect was observed in concentration of 100 ng/ml, there was significant difference as compared with control (p < 0.01). Anti-RAGE antibody partially inhibited the migration effect of HMGB1 on CD34(+) cells. It is concluded that the HMGB1 in certain concentration can enhance migration of CD34(+) cells, which may be mediated through RAGE.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Receptors, Immunologic
/
Signal Transduction
/
Cell Movement
/
Cells, Cultured
/
Antigens, CD34
/
Cell Biology
/
HMGB1 Protein
/
Fetal Blood
/
Receptor for Advanced Glycation End Products
Limits:
Female
/
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2009
Type:
Article
Similar
MEDLINE
...
LILACS
LIS