Comparison of migration characteristics of MSCs in different assay systems / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 404-407, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-302123
ABSTRACT
The aim of this study was to explore the difference of MSC migration mediated by SDF-1/CXCR4 axis through Boyden chamber in vitro migration assay. The SDF-1 density-dependence of MSC migration was observed. Subsequently, the effects of different blocking agents on hSDF-MSC migration were observed after MSC were treated with 50 nmol/L wortmannin, 10 micromol/L LY294002, 50 micromol/L PD98059, 10 micromol/L U73122, 126 micromol/L AMD3100 and 50 nmol/L verapamil respectively. The results showed the efficiency of MSC migration increased gradually with the increasing of hSDF-1 density. And after MSCs treatment with 50 nmol/L wortmannin, 10 micromol/L LY294002, 50 micromol/L PD98059, 10 micromol/L U73122 and 126 micromol/L AMD3100 respectively, the ability of MSC migration decreased. The ability of MSCs migration obviously decreased when MSCs were treated with U73122, AMD3100. It is concluded that the SDF-1/CXCR4-mediated MSC migration may be related to mitogen-activated protein kinase (MAPK), phosphatidylinositol phospholipase C (PI-PLC) and protein kinase (PKC) signal pathways.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Flavonoids
/
Protein Kinase C
/
Bone Marrow Cells
/
Signal Transduction
/
Cell Movement
/
Rats, Wistar
/
Receptors, CXCR4
/
Cell Biology
/
Mesenchymal Stem Cells
Limits:
Animals
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2009
Type:
Article
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