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Inhibition effect of gambogic acid on MUTZ-1 cell line and its possible mechanism / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 373-376, 2009.
Article in Chinese | WPRIM | ID: wpr-302130
ABSTRACT
This study was aimed to investigate the effects of gambogic acid on the cells of high-risk patients with myelodysplastic syndrome (MDS) in vitro and its mechanism. The inhibition effect of gambogic acid on growth of MUTZ-1 cell line of MDS-RAEB was detected by MTT method. Apoptosis and cell cycle were detected by morphological observation and flow cytometry respectively. The expressions of bax/bcl-2 gene at mRNA level were detected by RT-PCR. The results indicated that the Gambogic acid inhibited the growth of MUTZ-1 cells, the inhibitory rate of gambogic acid with the range of 0.2 - 0.8 microg/ml was enhanced along with increasing of drug concentration. Flow cytometric assay showed that the apoptotic rate of MUTZ-1 cells treated by gambogic acid also was enhanced along with increasing of drug concentration, the apoptotic rates resulting from gambogic acid (0, 0.4, 0.6, 0.8 microg/ml) were (5 +/- 0.5)%, (13 +/- 0.5)%, (37 +/- 0.7)% and (56 +/- 0.6)% respectively. The characteristic changes of apoptosis emerged in MUTZ-1 cells after being exposed to gambogic acid. Gambogic acid could significantly down-regulate the expressions of bcl-2 gene in a dose dependent manner, however, it had no effects on bax gene. It is concluded that within the range of concentration from 0.4 to 0. 8 microg/ml, gambogic acid can inhibit the growth of MUTZ-1 cells by inducing their apoptosis and down-regulating the expression of bcl-2 gene, which may be one of the main mechanisms underlying its antitumor effects.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / RNA, Messenger / Cell Line / Apoptosis / Proto-Oncogene Proteins c-bcl-2 / Xanthones / Bcl-2-Associated X Protein / Flow Cytometry / Genetics Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2009 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / RNA, Messenger / Cell Line / Apoptosis / Proto-Oncogene Proteins c-bcl-2 / Xanthones / Bcl-2-Associated X Protein / Flow Cytometry / Genetics Limits: Humans Language: Chinese Journal: Journal of Experimental Hematology Year: 2009 Type: Article