Effects of magnetic nanoparticle of fe(3)o(4) and 5-bromotetrandrine on apoptosis of K562/A02 leukemic cells induced by daunorubicin / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 54-59, 2009.
Article
in En
| WPRIM
| ID: wpr-302198
Responsible library:
WPRO
ABSTRACT
The aim of this study was to investigate the potential benefit of combination therapy with magnetic nanoparticle of Fe(3)O(4) and 5-Bromotetrandrine (5-BrTet) on chronic leukemia. The apoptosis was detected by flow cytometry (FCM), Wright staining and light microscope; the expressions of BAX and BCL-2 were measured by Western blot. The results showed that combination of daunorubicin (DNR) with either MNP (Fe(3)O(4)) or 5-BrTet exerted a potent cytotoxic effect on K562/A02 cells, while MNP (Fe(3)O(4)) and 5-BrTet co-treatment could synergistically enhance DNR-induced apoptosis. After treated with this regimen, the typical apoptotic morphological features were found in K562/A02 cells; the expression level of BCL-2 decreased and BAX increased markedly. It is concluded that MNP (Fe(3)O(4)) or 5-BrTet with DNR can induce apoptosis in K562/A02 cells, and they show distinct synergism when used together. The down-regulation of BCL-2 and the up-regulation of BAX may play important roles.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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Ferric Compounds
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Daunorubicin
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Down-Regulation
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Gene Expression Regulation, Leukemic
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Up-Regulation
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Apoptosis
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Proto-Oncogene Proteins c-bcl-2
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K562 Cells
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Benzylisoquinolines
Limits:
Humans
Language:
En
Journal:
Journal of Experimental Hematology
Year:
2009
Type:
Article