Protective immunity induced by the anti-idiotypic monoclonal antibody NP30 of Schistosoma japonicum / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 576-579, 2002.
Article
in English
| WPRIM
| ID: wpr-302248
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the protective immunity induced by the anti-idiotypic monoclonal antibody NP30 of Schistosoma japonicum in mice.</p><p><b>METHODS</b>An orthogonal table L(16) (4 x 2(12)) was selected as the experimental design. Eight-week-old Kunming outbred mice (male and female) were randomly divided into 16 experimental groups and 2 control groups. Control groups were injected with SP2/0 ascites intraperitoneally. Mice from each group were infected with 100 +/- 2 cercariae of Schistosoma japonicum in the abdominal skin and were sacrificed on the thirtieth day postchallenge. Adult worms were recovered and counted by perfusion of the left ventricle-portal vein. The SP2/0 ascites injected mice were used as controls and the percentage of protection was calculated.</p><p><b>RESULTS</b>Active immunization of mice with NP30 could produce protection levels ranging from 22.36% to 50.46% depending on the different immunity protocols. The best immunization protocol was established from the results.</p><p><b>CONCLUSIONS</b>Active immunization with NP30 can induce a degree of protection to infection with Schistosoma japonicum cercariae and NP30 is a potential vaccine candidate against Schistosoma japonicum.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Parasitology
/
Schistosoma mansoni
/
Schistosomiasis mansoni
/
Antibodies, Anti-Idiotypic
/
Analysis of Variance
/
Vaccination
/
Treatment Outcome
/
Animals, Outbred Strains
/
Therapeutic Uses
/
Allergy and Immunology
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2002
Type:
Article
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