Plating densities, alpha-difluoromethylornithine effects and time dependence on the proliferation of IEC-6 cells / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 518-520, 2002.
Article
in English
| WPRIM
| ID: wpr-302263
ABSTRACT
<p><b>OBJECTIVE</b>To characterize the role of plating densities and alpha-difluoromethylornithine (DFMO) on the proliferation of IEC-6 cells in vitro.</p><p><b>METHODS</b>IEC-6 cells were seeded in 96-well microplates at various densities in the presence or absence of DFMO. Cells were counted and their proliferative capability was monitored Days 1 to 7 with MTT assay at an optical density of 570 nm.</p><p><b>RESULTS</b>There was a positive relationship between cell number and OD value (r = 0.954, P < 0.01). Higher plating densities (> 0.5 x 10(4) cells/well) inhibited the growth of cells on Day 2. When the density reaches 4 x 10(4) cells/well, the OD value increased gradually and reached a peak on Day 5. After that, the OD value began to fall. The growth of IEC-6 cells was limited at a low density (0.2 x 10(4) cells/well) on Day 4. DFMO caused a complete inhibition of proliferation of IEC-6 cells on Days 1 to 3.</p><p><b>CONCLUSION</b>Proliferation of IEC-6 cells is related to plating density and incubation time. It is inhibited by DFMO, but is reversible when the incubation time is prolonged.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Time Factors
/
Cell Count
/
Cell Division
/
Cell Line
/
Eflornithine
/
Antineoplastic Agents
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2002
Type:
Article
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