Identification of a gene associated with astragalus and angelica's renal protective effects by silver staining mRNA differential display / 中华医学杂志(英文版)
Chin. med. j
; Chin. med. j;(24): 923-927, 2002.
Article
in En
| WPRIM
| ID: wpr-302273
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To identify genes associated with the chronic progression of renal disease and a stragalus and angelica (A&A)'s renal protective effects.</p><p><b>METHODS</b>The technique of silver staining mRNA differential display (DD) was used to investigate changes of gene expression in normal, sclerotic and A&A treated sclerotic kidneys. We isolated genes differentially expressed during the progression of renal disease which could be normalized by A&A.</p><p><b>RESULTS</b>Several genes related to A&A's protective effects were isolated and one of them was confirmed by Northern blot.</p><p><b>CONCLUSION</b>Silver staining mRNA differential display is a simple and effective technique for isolating differentially expressed genes. The isolated new gene may be related to the progression of chronic renal disease and contribute to A&A's protective effects.</p>
Full text:
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Index:
WPRIM
Main subject:
Pharmacology
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Staining and Labeling
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RNA, Messenger
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Drugs, Chinese Herbal
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Polymerase Chain Reaction
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Rats, Sprague-Dawley
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Protective Agents
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Angelica sinensis
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Astragalus Plant
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Drug Therapy
Limits:
Animals
Language:
En
Journal:
Chin. med. j
Year:
2002
Type:
Article