Preimplantation gender diagnosis by fluorescence in situ hybridization / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 874-877, 2002.
Article
in English
| WPRIM
| ID: wpr-302284
ABSTRACT
<p><b>OBJECTIVE</b>To describe the clinical application of fluorescence in situ hybridization (FISH ) in preimplantation gender diagnosis.</p><p><b>METHODS</b>Preimplantation gender diagnosis was performed in 2 female hemophilia A carriers, 1 male patient with glucose-6-phosphate dehydrogenase (G-6-PD) deficiency and 2 male patients with Y chromosome abnormality. Embryo sex was identified by FISH in total of 6 treatment cycles.</p><p><b>RESULTS</b>A total of 123 cumulus-oocytes were retrieved in 6 treatment cycles. Sixty-one embryos were available for embryo biopsy. The success rate of biopsy was 86.9% (53/61), with a further cleavage rate of 62.3% (33/53). In the FISH procedure, one cell was lost during fixation, leading to a 98.1% (52/53) fixation rate. Totally, 16 female embryos and 3 male embryos were transferred to 5 patients in 6 cycles. Three healthy babies were born. The diagnosis was confirmed by subsequent analysis of amniocytes and embryonic buds after embryo reduction.</p><p><b>CONCLUSIONS</b>FISH is an efficient and reliable technique for determining the sex of human preimplantation embryos. Selective abortion and births of affected children can be avoided by preimplantation gender diagnosis.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sex Determination Analysis
/
Biopsy
/
Blastocyst
/
In Situ Hybridization, Fluorescence
/
Amniocentesis
Limits:
Adult
/
Female
/
Humans
/
Male
/
Pregnancy
Language:
English
Journal:
Chinese Medical Journal
Year:
2002
Type:
Article
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