Cell-specific expression of the diphtheria toxin A-chain coding sequence induces cancer cell suicide / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 869-873, 2002.
Article
in En
| WPRIM
| ID: wpr-302285
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To test whether the diphtheria toxin A (DT-A) chain coding sequence linked to murine immunoglobulin Kappa light chain (IgKappa) promoter and enhancer have selective cytocidal effects on IgKappa producing cells.</p><p><b>METHODS</b>The diphtheria toxin A gene or beta galactosidase (beta-gal) gene were linked to a murine IgKappa promoter and enhancer to construct pcDNA3IgKappaDTA or pcDNA3IgKappaLacZ plasmids. These plasmids were transfected into IgKappa producing or non-producing cells by the liposome coated DNA method. Expression of beta-gal activity and effects on cell growth of transfected cells were assessed.</p><p><b>RESULTS</b>The beta-gal gene, under the control of cytomegalovirus (CMV) promoter, can express in all cell lines. Expression of beta-gal under the control of the IgKappa promoter was detected only in the IgKappa producing cell line, CA46. Expression of beta-gal was greatly suppressed when cotransfected with pcDNA3IgKappaDTA in CA46 cells. Cell growth of CA46 cells transfected with pcDNA3IgKappaDTA plasmid was significantly inhibited compared with CA46 cells transfected with pcDNA3IgKappaLacZ.</p><p><b>CONCLUSION</b>Selective killing of IgKappa producing cells can be attained by introducing the diphtheria toxin A gene under the control of IgKappa promoter and enhancer.</p>
Full text:
1
Index:
WPRIM
Main subject:
Peptide Fragments
/
Therapeutics
/
Genes, Immunoglobulin
/
Tumor Cells, Cultured
/
Genetic Therapy
/
Enhancer Elements, Genetic
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Promoter Regions, Genetic
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Immunoglobulin kappa-Chains
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Diphtheria Toxin
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Genetics
Limits:
Humans
Language:
En
Journal:
Chinese Medical Journal
Year:
2002
Type:
Article