Effect of different irradiation doses on the establishment of murine cGVHD model after MHC matched spleen stem cell transplantation / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1115-1119, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-302337
ABSTRACT
This study was aimed to investigate the effect of different irradiation doses on the establishment of murine cGVHD model after MHC matched spleen stem cell transplantation. The male mouse BALB/c(H)-2d was totally irradiated with different radiation dose of (60)Co (TBI), then was infused with the same number of splenocytes from MHC matched DBA/2 male mice. After transplantation, the bodyweight, general appearance, hair changes, survival time and pathological damage were observed. The results indicated that compared to the control group (0 Gy) and the 7.0 Gy group, the mice irradiated with 7.5 Gy and 8.0 Gy showed cGVHD symptoms and obvious pathological damage. At the end of experiments (60 d after transplantation), all mice irradiated by 7.5 Gy survived while only 60% animals survived in the 8.0 Gy group. It is concluded that under infusion of 10(8) MHC matched splenocytes per mouse, 7.5 Gy irradiation is appropriate to efficiently establish cGVHD model. This study laid an important foundation for further studying the pathogenesis, biological characteristics, and intervention factors of cGVHD.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Radiation Dosage
/
Radiation Effects
/
Spleen
/
Transplantation, Homologous
/
Whole-Body Irradiation
/
Transplantation Conditioning
/
Cell Biology
/
Stem Cell Transplantation
/
Disease Models, Animal
/
Graft Survival
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2014
Type:
Article
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