The expression of proto-oncogene c-erbB₂ and its role in the initiation of primordial follicle growth in rat ovary / 生理学报
Acta Physiologica Sinica
;
(6): 424-430, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-302432
ABSTRACT
Little is known about the factors that control the initiation of growth of primordial follicles. The objective of the present study was to investigate the effect of c-erbB₂ on the onset of primordial follicle development, and whether c-erbB₂ mediates the effect of epidermal growth factor (EGF) in this process. We synthesized three pairs of siRNAs targeting the c-erbB₂ mRNA and transferred them into the newborn rat ovary cultured in vitro with Metafectene. After siRNAs transfection, the efficiency of siRNAs was tested by examining c-erbB₂ mRNA and protein levels. The level of c-erbB₂ mRNA was reduced by 49.6%, 46.7% and 82.6% respectively after transfecting siRNA1, siRNA2 and siRNA3, and the level of ErbB₂ protein also reduced remarkably after siRNA3 transfection. c-erbB₂ siRNA3 significantly inhibited the primordial follicle initiation and development; EGF augmented primordial follicles formation, but the effect was abolished by c-erbB₂ siRNA3. All of these results suggest that c-erbB₂ plays an important role in primordial follicle development and folliculogenesis initiation, and mediates the effect of EGF on primordial follicle development.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Organ Culture Techniques
/
Physiology
/
Receptor, ErbB-2
/
RNA, Small Interfering
/
Ovarian Follicle
/
Animals, Newborn
Limits:
Animals
Language:
Chinese
Journal:
Acta Physiologica Sinica
Year:
2009
Type:
Article
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