Effects of Rho/ROCK signal pathway on AGEs-induced morphological and functional changes in human dermal microvascular endothelial cells / 生理学报
Acta Physiologica Sinica
;
(6): 132-138, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-302470
ABSTRACT
The present study aimed to determine the role of Rho/Rho kinase (Rho/ROCK) phosphorylation on advanced glycation end products (AGEs)-induced morphological and functional changes in human dermal microvascular endothelial cells (HMVECs). HMVECs were respectively incubated with different concentrations of AGEs-modified human serum albumin (AGEs-HSA) for different time. In some other cases, HMVECs were pretreated with ROCK inhibitors (H-1152 or Y-27632). The morphological changes of F-actin cytoskeleton were visualized by rhodamine-phalloidin staining and the phosphorylation of Rho and ROCK were determined by Western blot. Endothelial monolayer permeability was assessed by measuring the flux of FITC-albumin across the endothelial cells. The results showed that the distribution of F-actin was significantly altered by AGEs-HSA in time and dose-dependent patterns. These effects were inhibited by ROCK inhibitors. The phosphorylation of Rho and RCOK was remarkably increased by AGEs-HSA treatment while total Rho and ROCK protein levels were not affected. The permeability of endothelial monolayer was dramatically increased by AGEs-HSA, and both ROCK inhibitors (H-1152 or Y-27632) attenuated these hyperpermeability responses. The results obtained suggest that the phosphorylation of Rho/ROCK plays an important role in AGEs-induced morphological and functional alterations in HMVECs.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phalloidine
/
Pharmacology
/
Phosphorylation
/
Pyridines
/
Rhodamines
/
Actin Cytoskeleton
/
Serum Albumin
/
Endothelium, Vascular
/
Signal Transduction
/
Actins
Limits:
Humans
Language:
Chinese
Journal:
Acta Physiologica Sinica
Year:
2009
Type:
Article
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