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Slow rise of intracellular Ca(2+) concentration in rat primary sensory neurons triggered by loureirin B / 生理学报
Acta Physiologica Sinica ; (6): 115-120, 2009.
Article in English | WPRIM | ID: wpr-302473
ABSTRACT
In the present study, the intracellular free calcium concentration ([Ca(2+)](i)) in acutely isolated rat dorsal root ganglia (DRG) neurons modulated by loureirin B, an active component of "dragon's blood" which is a kind of Chinese herbal medicine, was determined by the means of Fura-2 based microfluorimetry. It was found that loureirin B could evoke the elevation of [Ca(2+)](i) in a dose-dependent manner. However, the elevation of [Ca(2+)](i) evoked in the calcium free solution was much smaller than that in the standard external cell solution, suggesting that most change of [Ca(2+)](i) was generated by the influx of extracellular Ca(2+), not by the activities of intracellular organelles like Ca(2+) stores and mitochondria. In addition, the mixture of loureirin B and caffeine also induced [Ca(2+)](i) rise, but the peak of [Ca(2+)](i) rise induced by the mixture was significantly lower than that by caffeine alone, which means the triggering pathway and the targets of caffeine are probably involved in loureirin B-induced [Ca(2+)](i) rise. Moreover, compared to the transients induced by caffeine, KCl and capsaicin, the loureirin B-induced [Ca(2+)](i) rise is much slower and more stable. These results indicate that the capability of loureirin B of inducing the [Ca(2+)](i) rise is solid and unique.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Resins, Plant / Caffeine / Calcium / Ganglia, Spinal / Metabolism / Neurons, Afferent Limits: Animals Language: English Journal: Acta Physiologica Sinica Year: 2009 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Resins, Plant / Caffeine / Calcium / Ganglia, Spinal / Metabolism / Neurons, Afferent Limits: Animals Language: English Journal: Acta Physiologica Sinica Year: 2009 Type: Article