Overexpression of PDZ1 domain of PSD-95 protein rescues hippocampal neurons from apoptosis induced by oxygen-glucose deprivation / 生理学报
Acta Physiologica Sinica
;
(6): 730-736, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-302497
ABSTRACT
To detect the effect of PDZ1, domain of postsynaptic density 95 (PSD-95), on apoptosis of hippocampal neurons induced by oxygen-glucose deprivation (OGD), Sprague-Dawley rat hippocampal neurons were infected with PDZ1-viruses after 21 days of plating. Twenty-four hours after infection, cells were treated with OGD for 1.5 h, then were incubated with DAPI and apoptosis-like cells were characterized, or were collected for co-immunoprecipitation and Western blot analyses. The results showed that (1) PDZ1 overexpression was observed in hippocampal neurons; (2) Apoptosis induced by OGD was obviously decreased in neurons overexpressing PDZ1 (P<0.05); (3) Overexpression of PDZ1 prevented the binding of GluR6 to PSD-95; (4) Overexpression of PDZ1 inhibited MLK3 and JNK1/2 activation induced by OGD. These results indicate that overexpression of PDZ1 may prevent hippocampal neurons from apoptosis induced by OGD.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxygen
/
Pathology
/
Cells, Cultured
/
Chemistry
/
Rats, Sprague-Dawley
/
Apoptosis
/
Culture Media
/
Cell Biology
/
Intracellular Signaling Peptides and Proteins
/
PDZ Domains
Limits:
Animals
Language:
Chinese
Journal:
Acta Physiologica Sinica
Year:
2008
Type:
Article
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