Interferon regulatory factor-1 exerts inhibitory effect on neointimal formation after vascular injury / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 91-96, 2009.
Article
in English
| WPRIM
| ID: wpr-302642
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of interferon regulatory factors (IRFs) on neointimal formation after vascular injury in the mouse, and its possible mechanism.</p><p><b>METHODS</b>Vascular injury was induced by polyethylene cuff placement around the left femoral artery of IRF-1-deficient mice and C57BL/6J mice. The mRNA expressions of IRF-1, IRF-2, angiotensin II type 2 (AT2) receptor, interleukin-1 beta converting enzyme (ICE), inducible nitric oxide synthase (iNOS) were detected by RT-PCR and immunohistochemical staining.</p><p><b>RESULTS</b>Neointimal formation after vascular injury was significantly greater in IRF-1-deficient mice than that in C57BL/6J mice (P<0.05). In contrast, TUNEL-positive nuclei to total nuclei in the neointima and media in vascular smooth muscle cell (VSMC) in the injured artery significantly attenuated in IRF-1-deficient mice compared to C57BL/6J mice (P<0.05). The expressions of AT2 receptor as well as pro-apoptotic genes such as ICE and iNOS in C57BL/6J mice were up-regulated in response to vascular injury, but this upregulation was attenuated in IRF-1-deficient mice.</p><p><b>CONCLUSIONS</b>Our results suggest that IRF-1 induces VSMC apoptosis and inhibits neointimal formation after vascular injury at least partly due to the upregulation of AT2 receptor, ICE and iNOS expressions. These results indicate that IRF-1 exerts an inhibitory effect on neointimal formation through the induction of apoptosis in VSMCs.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Tunica Intima
/
Apoptosis
/
Mice, Knockout
/
Platelet Endothelial Cell Adhesion Molecule-1
/
Caspase 1
/
Cell Biology
/
Receptor, Angiotensin, Type 2
/
Nitric Oxide Synthase Type II
Limits:
Animals
Language:
English
Journal:
Chinese Medical Sciences Journal
Year:
2009
Type:
Article
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