Neurogenesis by activation of inherent neural stem cells in the rat hippocampus after cerebral infarction / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 41-45, 2009.
Article
in English
| WPRIM
| ID: wpr-302652
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the changes of neural stem cells (NSCs) in the rat hippocampus after cerebral infarction (CI) and to evaluate the neurogenesis caused by the activation of NSCs.</p><p><b>METHODS</b>CI models of rats were made and rats were assigned to 6 groups sham-operated, 1 day, 3 days, 7 days, 14 days, and 28 days after CI. The dynamic expression of bromodeoxyuridine (BrdU), polysialylated neural cell adhesion molecule (PSA-NCAM), glial fibrillary acidic protein (GFAP), and neuronal nuclear antigen (NeuN) were determined by immunohistochemistry and immunofluorescence staining. BrdU was used to mark the proliferated NSCs. PSA-NCAM was used to mark the plasticity of activated NSCs. GFAP and NeuN were used to mark the differentiated NSCs.</p><p><b>RESULTS</b>Compared with the controls, the number of BrdU+ cells in the hippocampus increased significantly at 1 day after CI (P < 0.05), reached peak at 7 days after CI (P < 0.05), decreased but still elevated compared with the controls at 14 days after CI (P < 0.05), and nearly unchanged at 28 days after CI. The number of BrdU+/PSA-NCAM+ cells increased significantly at 7 days after CI (P < 0.05), reached peak at 14 days after CI (P < 0.05), and decreased but still elevated compared with the controls at 28 days after CI (P < 0.05). The number of BrdU+/PSA-NCAM+ cells was equal to 60% of the number of BrdU+ cells in all the same period. The number of BrdU+/NeuN+ cells in the hippocampus increased significantly at 14 days after CI (P < 0.05) and reached peak at 28 day after CI (P < 0.05). The number of BrdU+/GFAP+ cells in the hippocampus nearly unchanged after CI.</p><p><b>CONCLUSION</b>CI can stimulate the proliferation of inherent NSCs, and most proliferated NSCs may differentiate into neurons and represent neural plasticity.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Sialic Acids
/
Bromodeoxyuridine
/
Cell Nucleus
/
Cerebral Infarction
/
Rats, Wistar
/
Dentate Gyrus
/
Cell Biology
/
Neural Cell Adhesion Molecule L1
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Chinese Medical Sciences Journal
Year:
2009
Type:
Article
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