Oxidized high-density lipoprotein promotes maturation and migration of bone marrow derived dendritic cells from C57BL/6J mice / 中国医学科学杂志(英文版)
Chinese Medical Sciences Journal
;
(4): 224-229, 2008.
Article
in English
| WPRIM
| ID: wpr-302666
ABSTRACT
<p><b>OBJECTIVE</b>To explore the influence of oxidized high-density lipoprotein (oxHDL) on the maturation and migration of bone marrow-derived dendritic cells (BMDCs) from C57BL/6J mice.</p><p><b>METHODS</b>The C57BL/6J mice bone marrow cell suspension was prepared and purified. Recombinant granulocyte-macrophage colony-stimulating factor (rmGM-CSF) and recombinant interleukin-4 (rmIL-4) were used to promote monocytes to differentiate and suppress lymphocytes. Then 50 microg/mL oxHDL was added to stimulate BMDCs, using 50 microg/mL high-density lipoprotein (HDL) as homologous protein control, PBS as negative control, and 1 microg/mL lipopolysaccharide (LPS) as positive control. The CD86 and MHCII expression rates were detected with fluorescence-activated cell sorting (FACS). Liquid scintillation counting (LSC) was used in mixed lymphocyte reactions (MLRs) to reflect the ability of BMDCs in stimulating the proliferation of homologous T cells. Levels of cytokines IL-12 and IL-10 were detected by ELISA. The cell migration was evaluated with the transwell system.</p><p><b>RESULTS</b>Compared with PBS group, the expressions of CD86 and MHCII, counts per minute of MLRs, secretion of IL-12 and IL-10, and number of migrated cells in oxHDL group and LPS group significantly increased (all P<0.05), while the increment was less in oxHDL group than LPS group. The number of migrated cells in oxHDL group was about twice of that in HDL group.</p><p><b>CONCLUSION</b>OxHDL may promote the maturation and migration of BMDCs in vitro.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Dendritic Cells
/
Bone Marrow Cells
/
Cell Differentiation
/
Cell Movement
/
Cells, Cultured
/
Cell Biology
/
Lipoproteins, HDL
/
Lipoproteins, LDL
Limits:
Animals
/
Humans
Language:
English
Journal:
Chinese Medical Sciences Journal
Year:
2008
Type:
Article
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