Comparison of the pharmacokinetics of lidamycin in mice determined by two methods / 药学学报
Acta Pharmaceutica Sinica
;
(12): 695-699, 2004.
Article
in English
| WPRIM
| ID: wpr-302734
ABSTRACT
<p><b>AIM</b>To compare two methods, the total radioactivity assay (RA method) and the radioactivity assay after separation with high performance liquid chromatography (HPLC-RA method).</p><p><b>METHODS</b>125I-Lidamycin was prepared by Iodogen method and separated by size exclusive high performance liquid chromatography. The pharmacokinetic parameters of lidamycin were assayed by two methods after intravenous injection to mice at the dose of 100 microg x kg(-1), and compared by statistical analysis.</p><p><b>RESULTS</b>The pharmacokinetic parameters (Vd, T1/2alpha, T1/2beta, K21, K10, K12, AUC and CL) showed significant difference between the two methods (P < 0.05).</p><p><b>CONCLUSION</b>The HPLC-RA method was better than the RA method to determine unchanged 125I-lidamycin.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
Pharmacokinetics
/
Chromatography, High Pressure Liquid
/
Sensitivity and Specificity
/
Area Under Curve
/
Enediynes
/
Aminoglycosides
/
Iodine Radioisotopes
/
Isotope Labeling
/
Methods
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
Acta Pharmaceutica Sinica
Year:
2004
Type:
Article
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