Effect of ginsenoside Rg1 on expression of p21, cyclin E and CDK2 in the process of cell senescence / 药学学报
Acta Pharmaceutica Sinica
;
(12): 673-676, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-302739
ABSTRACT
<p><b>AIM</b>To explore the possible role of p21, cyclin E and cyclin-dependent kinase 2 (CDK2) in the protection of ginsenoside Rg1 against tert-butylhydroperoxide (t-BHP)-induced senescence in WI-38 cells.</p><p><b>METHODS</b>The cellular ultrastructure, cytometric assay and beta-galactosidase (beta-gal) cytochemistry staining were used to evaluate cell senescence. The levels of of p21, cyclin E and CDK2 protein were detected by Western blot.</p><p><b>RESULTS</b>Pretreatment with Rg1 significantly attenuated t-BHP-induced senescence in WI-38 cells. Simultaneously, compared with cells treated with t-BHP alone, Rg1 pretreatment markedly decreased the level of p21 protein and increased the levels of CDK2 and cyclin E.</p><p><b>CONCLUSION</b>p21, cyclin E and CDK2 may be involved in the process of ginsenoside Rg1 protection against t-BHP-induced senescence in WI-38 cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Plants, Medicinal
/
Cell Line
/
Chemistry
/
Proto-Oncogene Proteins p21(ras)
/
Cellular Senescence
/
Cyclin E
/
Tert-Butylhydroperoxide
/
Cell Biology
/
Ginsenosides
Limits:
Humans
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2004
Type:
Article
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