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Application of PSD-MALDI-TOF-MS to protein identification / 药学学报
Acta Pharmaceutica Sinica ; (12): 627-630, 2004.
Article in Chinese | WPRIM | ID: wpr-302748
ABSTRACT
<p><b>AIM</b>To identify protein spots on two dimentional protein electrophoresis (2DE) by post-source decay (PSD) technique associated with library search.</p><p><b>METHODS</b>The PSD-MALDI-TOF-MS method was set up by a segment of ACTH and a peptide digested by trypsin for TPA.</p><p><b>RESULTS</b>Two unknown protein spots on 2DE were identified as 40S ribosomal protein S12 and dnaK suppressor protein separately by established PSD-MALDI-TOF-MS method.</p><p><b>CONCLUSION</b>PSD technique has greater application prospects in peoteomics.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Peptide Fragments / Ribosomal Proteins / Peptide Mapping / Molecular Sequence Data / Electrophoresis, Gel, Two-Dimensional / Chemistry / Amino Acid Sequence / Tissue Plasminogen Activator / Adrenocorticotropic Hormone / Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Type of study: Diagnostic study Language: Chinese Journal: Acta Pharmaceutica Sinica Year: 2004 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Peptide Fragments / Ribosomal Proteins / Peptide Mapping / Molecular Sequence Data / Electrophoresis, Gel, Two-Dimensional / Chemistry / Amino Acid Sequence / Tissue Plasminogen Activator / Adrenocorticotropic Hormone / Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Type of study: Diagnostic study Language: Chinese Journal: Acta Pharmaceutica Sinica Year: 2004 Type: Article