Application of PSD-MALDI-TOF-MS to protein identification / 药学学报
Acta Pharmaceutica Sinica
;
(12): 627-630, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-302748
ABSTRACT
<p><b>AIM</b>To identify protein spots on two dimentional protein electrophoresis (2DE) by post-source decay (PSD) technique associated with library search.</p><p><b>METHODS</b>The PSD-MALDI-TOF-MS method was set up by a segment of ACTH and a peptide digested by trypsin for TPA.</p><p><b>RESULTS</b>Two unknown protein spots on 2DE were identified as 40S ribosomal protein S12 and dnaK suppressor protein separately by established PSD-MALDI-TOF-MS method.</p><p><b>CONCLUSION</b>PSD technique has greater application prospects in peoteomics.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptide Fragments
/
Ribosomal Proteins
/
Peptide Mapping
/
Molecular Sequence Data
/
Electrophoresis, Gel, Two-Dimensional
/
Chemistry
/
Amino Acid Sequence
/
Tissue Plasminogen Activator
/
Adrenocorticotropic Hormone
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2004
Type:
Article
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