Determination of five components in compound hypotensive tablet by HPLC / 药学学报

ABSTRACT

<p><b>AIM</b>To establish a method for the determination of the five components (reserpine, chlordiazepoxide, hydrochlorothiazide, dihydralazine sulfate, triamterene) in compound hypotensive tablet.</p><p><b>METHODS</b>The chromatography was performed using a CN column with acetontrile-0.1 mol L(-1) sodium heptasulfonate solution (73) and (55) as the mobile phases. The detection wavelength was 267 nm for reserpine, chlordiazepoxide and hydrochlorothiazide, 310 nm for dihydralazine sulfate, 360 nm for triamterene.</p><p><b>RESULTS</b>The linear range of each component was tested, and the recovery and stability of each component was satisfactory, three lots of samples were determined using the method.</p><p><b>CONCLUSION</b>This is an accurate and credible quality control method for compound hypotensive tablet.</p>