Adenovirus mediated IL-24 gene expression inhibits growth of human glioma cell in vitro / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 279-286, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-302823
ABSTRACT
To investigate the inhibitory effect and anti-cancer mechanism of adenovirus mediated IL-24 gene expression on the human U251 glioma cell. U251 glioma cells were infected with Ad-IL-24 at various multiplicity of infection (MOIs). Cell proliferation was determined by MTT assay. Cell apoptosis was detected by flow cytometry and Hochest staining. The transcription of apoptosis-related genes was analyzed by reverse transcription-PCR (RT-PCR), and the expression of Cleaved Caspase-3 was analyzed by Western blotting. The result showed that the growth of U251 glioma cells was significantly inhibited by Ad-IL-24 at the MOI of 100. The apoptotic rate of U251 glioma cells was 42% 72 h after infection with Ad-IL-24. Four days after infection, the growth of the U251 glioma cells was inhibited to 50%. RT-PCR showed that Ad-IL-24 not only up-regulated expression of bax/bcl-2, ICE, C-myc, p53 and down-regulated the expression of HIF-1alpha, but also enhanced Caspase-3 activation, eventually resulting apoptosis. Taken together, these results suggest that infection of U251 glioma cells with Ad-IL-24 can inhibit growth and induce apoptosis significantly by the regulation of apoptosis-related genes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Recombination, Genetic
/
Brain Neoplasms
/
Tumor Cells, Cultured
/
Genetic Therapy
/
Adenoviridae
/
Interleukins
/
Apoptosis
/
Cell Proliferation
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2009
Type:
Article
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