Purification of recombinant lysostaphin by monoclonal antibody affinity chromatography / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 147-151, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-302842
ABSTRACT
Lysostaphin, a specific endopeptidase enzyme derived from Staphylococcus aureus, is a bactericidal agent against Staphylococcus and difficult to be drug-resistant. This study established the monoclonal antibody affinity chromatography to obtain lysostaphin of high purity for drug-use standard. The purified Lysostaphin was of > 95% purity and its recovery rate more than 90%. Moreover, the affinity column kept its efficiency of purification invariable after more than 30 times repeat. Also, the dye release assay validated that the purified lysostaphin had significant bactericidal activity. This method was simple and of high efficacy for the lysostaphin purification and showed its potency in commercial production.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Staphylococcus aureus
/
Recombinant Proteins
/
Chromatography, Affinity
/
Allergy and Immunology
/
Lysostaphin
/
Methods
/
Antibodies, Monoclonal
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2009
Type:
Article
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