Construction and activity of recombinant adeno-associated virus expressing vasostatin / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1949-1954, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-302886
ABSTRACT
Vasostatin, a 180-amino acid fragment from the N-terminal domain of calreticulin, is a potent endogenous angiogenesis inhibitor, which can inhibit the growth of many kinds of experimental tumor. But a recent report that vasostatin can enhance the malignant behavior of neuroendocrine tumor reminds us to be cautious to develop it as an anti-tumor medicine. VAS cDNA was cloned into pAAV-2 expression vector; recombinant virus rAAV-VAS was generated by a three plasmids, helper free packaging method. MS1 mouse pancreatic endothelial cell and human colon tumor HCT-116 cell were infected with rAAV-VAS. Transgene expression was analyzed by Western blotting analysis; cell proliferation was determined by MTT assay. The therapeutic potential of rAAV-VAS was evaluated in subcutaneous HCT-116 xenograft mouse model. rAAV-VAS inhibited the proliferation of MS1 but not HCT-116 cell. HCT-116 cell infected with rAAV-VAS secreted VAS protein into the supernatant effectively. The intratumoral delivery of rAAV-VAS inhibited the xenograft growth and microvessel density in tumors significantly. Our results show the effectiveness of rAAV-VAS as an angiogenesis inhibitor in suppressing tumor growth, validating the application of rAAV-VAS gene therapy in treatment against colon cancer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Peptide Fragments
/
Pharmacology
/
Recombinant Proteins
/
Tumor Cells, Cultured
/
Colonic Neoplasms
/
Dependovirus
/
Angiogenesis Inhibitors
/
Calreticulin
/
Cell Proliferation
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2008
Type:
Article
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