Establishment of human IFN-gamma in vitro release assay and its application in tuberculosis diagnosis / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1653-1657, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-302905
ABSTRACT
This study aimed to establish human IFN-gamma (hIFN-gamma) in vitro release assay and to apply it in diagnosis of human tuberculosis. Human IFN-gamma gene was cloned and expressed in Escherichia coli. The recombinant hIFN-gamma was purified and used as immunogen to immunize mice and rabbits respectively. Monoclonal and polyclonal antibodies were respectively developed and a sandwich ELISA was established. The heparized whole blood from 111 active tuberculosis patients and 292 clinical healthy controls were collected. The blood was stimulated with tuberculosis specific fused antigen ESAT-6/CFP-10 and the plasma was collected for IFN-gamma detection. The sensitivity for tuberculosis diagnosis was 95.5%, whereas the positive detection rate for the healthy controls was 16.7%. There was a significant difference between the patients and healthy controls (P<0.01) indicating that this assay had a high sensitivity and specificity, and thus could be promising in tuberculosis diagnosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tuberculosis
/
Enzyme-Linked Immunosorbent Assay
/
T-Lymphocytes
/
Interferon-gamma
/
Bodily Secretions
/
Diagnosis
/
Allergy and Immunology
/
Mice, Inbred BALB C
/
Antibodies, Monoclonal
/
Mycobacterium tuberculosis
Type of study:
Diagnostic study
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Biotechnology
Year:
2008
Type:
Article
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