A preliminary study on genome-wide expression profiling of laryngeal squamous cell carcinoma / 中华耳鼻咽喉头颈外科杂志
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
;
(12): 232-235, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-302966
ABSTRACT
<p><b>OBJECTIVE</b>To identify the genes differentially expressed and underlying molecular mechanism in laryngeal squamous cell carcinoma by using cDNA microarrays.</p><p><b>METHODS</b>Using Illumina Human HT-12 BeadChip, gene expressions were detected in ten pairs of laryngeal cancer tissues and adjacent normal tissues. Total RNA was extracted and reverse-transcribed into cDNA. Labeled cDNA were hybridized with cDNA microarray, data were read and images were scanned. All the samples had passed quality control testing.</p><p><b>RESULTS</b>Through Illumina Genomstudio 1.9.0 Data processing software (P < 0.05 or diffscore>13, diffscore<-13) and multiple displacement t test(FDR<0.05), 426 genes showed statistically significant differences in expressions between laryngeal tumor tissues and corresponding adjacent normal tissues, with 222 up-regulated genes and 204 down-regulated genes in laryngeal cancer tissues. These up- or down-regulated genes were indicated to involve in cellular processes relevant to the cancer phenotype, such as proliferation, cell cycle, chromosome segregation, mitosis and meiosis. These differentially expressed genes also took part in cancer related signaling pathways as well, for instance, metabolic pathways, cell cycle, DNA replication, glutathione metabolism, mucin type O-Glycan biosynthesis, drug metabolism-cytochrome P450 and so on.</p><p><b>CONCLUSION</b>The set of genes identified here and their functional annotations contribute to a better understanding of the pathogenesis of laryngeal squamous cell carcinoma from the view of multiple gene interactions and provide candidate markers for improving diagnosis, prognosis and treatment of this cancer.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Carcinoma, Squamous Cell
/
Gene Expression
/
Gene Expression Regulation, Neoplastic
/
Laryngeal Neoplasms
/
Oligonucleotide Array Sequence Analysis
/
Gene Expression Profiling
/
Genome-Wide Association Study
/
Genetics
/
Head and Neck Neoplasms
Limits:
Aged
/
Aged80
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Otorhinolaryngology Head and Neck Surgery
Year:
2014
Type:
Article
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