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Acupuncture-serum decreases Ca2+ content in cultured rat myocardial cells / 中国针灸
Chinese Acupuncture & Moxibustion ; (12): 367-370, 2006.
Article in Chinese | WPRIM | ID: wpr-303065
ABSTRACT
<p><b>OBJECTIVE</b>To explore whether humoral factors play a role in the mechanisms of acupuncture.</p><p><b>METHODS</b>Primary culture of myocardial cells of neonatal rats were carried out. Five days later, they were labeled by fluorescent molecular probe Fluo-3AM. Changes of Ca2+ contents in the cultured myocardial cells after addition of the normal rat serum or acupuncture-serum of the rat who received acupuncture at "Neiguan" (PC 6) and Jianshi (PC 5), were dynamically observed by a confocal laser scanning microscopy.</p><p><b>RESULTS</b>After addition of normal serum of the rat, the intracellular Ca2+ level increased to a certain degree, and then gradually tended to stability, which was significantly decreased by addition of the acupuncture-serum (P < 0.01).</p><p><b>CONCLUSION</b>The serum of the rat who received acupuncture at acupoints can decrease the Ca2+ level in cultured myocardial cells, which provides a direct evidence for serum factors involving in acupuncture mechanism.</p>
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Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Acupuncture Therapy / Cells, Cultured / Chemistry / Calcium / Rats, Wistar / Rats, Sprague-Dawley / Myocytes, Cardiac / Serum Limits: Animals Language: Chinese Journal: Chinese Acupuncture & Moxibustion Year: 2006 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Acupuncture Therapy / Cells, Cultured / Chemistry / Calcium / Rats, Wistar / Rats, Sprague-Dawley / Myocytes, Cardiac / Serum Limits: Animals Language: Chinese Journal: Chinese Acupuncture & Moxibustion Year: 2006 Type: Article