Effect of lysyl oxidase on migration and adhesion of human gastric cancer HGC-27 cells in vitro / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 331-333, 2011.
Article
in Zh
| WPRIM
| ID: wpr-303303
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of lysyl oxidase (LOX) on the migration and adhesion of the human gastric cancer cell line HGC-27 cells in vitro.</p><p><b>METHODS</b>The human gastric cancer cell line HGC-27 cells were cultured in vitro, and treated with different concentration of β-aminopropionitrile (BAPN). The ability of migration was assessed by wound-healing assay. The ability of adhesion was detected by homogenous and heterogeneous adhesion experiments.</p><p><b>RESULTS</b>Compared that with 0 mmol/L BAPN, the ability of migration of the cells after treatment with 0.2 mmol/L BAPN was descended at 8, 24, 32 and 48 h; the number of cells with homogeneous adhesion was increased from (6.97 ± 0.07) × 10(3)/ml to (7.78 ± 0.11) × 10(3)/ml; and the number of cells with heterogeneous adhesion was decreased from (8.98 ± 0.15) × 10(3)/ml to (8.35 ± 0.10) × 10(3)/ml, both < 0.05. Compared with that of cells treated with 0 mmol/L and 0.2 mmol/L BAPN, the migration ability of cells after treatment with 0.3 mmol/L BAPN was descended at 8, 24, 32 and 48 h; the number of cells with homogeneous adhesion was raised to (8.02 ± 0.11) × 10(3)/ml and the number of cells with heterogeneous adhesion was down to (7.93 ± 0.07) × 10(3)/ml (P < 0.05).</p><p><b>CONCLUSION</b>LOX may promote the metastasis of cancer cells by enhancing invasion, increasing heterogeneous adhesion and decreasing homogeneous adhesion.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Pharmacology
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Physiology
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Stomach Neoplasms
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Cell Adhesion
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Cell Movement
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Cell Line, Tumor
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Dose-Response Relationship, Drug
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Aminopropionitrile
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Protein-Lysine 6-Oxidase
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Oncology
Year:
2011
Type:
Article