Study on the mechanism of protective effect of oral L-arginine on intestine after scald injury in rats / 中华烧伤杂志
Chinese Journal of Burns
;
(6): 259-261, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-303655
ABSTRACT
<p><b>OBJECTIVE</b>To explore the mechanism of protective effect of oral L-arginine (L-Arg) on the intestine after scald injury in rats.</p><p><b>METHODS</b>Sixty-six Sprague-Dawley (SD) rats were randomly divided into three groups i.e. normal control (N, n = 6, without treatment), oral L-arginine group (A, n = 30, with 1 ml 70 g/L of L-Arg per os 2 times a day from 2 post scald hour (PSH)) on with normal enteral feeding and group B (n = 30, with oral feeding of cold boiled water after scald). The changes in the content of superoxide dismutase (SOD), malondialdehyde (MDA), nitric oxide (NO), endothelin (ET), ET/NO ratio in the intestine and the level of plasma endotoxin (LPS) in portal vein were assessed at 6, 12, 24, 48, 72 PSH. Ileum tissue samples were harvested for pathological examination.</p><p><b>RESULTS</b>The ET content in the intestinal tissue in A group at 6, 12 and 24 PSH (0.80 +/- 0.26 ng/g, 0.75 +/- 0.30 ng/g, 0.63 +/- 0.22 ng/g) was obviously lower than that in B group (1.26 +/- 0.38 ng/g, 1.34 +/- 0.37 ng/g, 0.97 +/- 0.19 ng/g, P < 0.05), but the NO contents in the intestine in A group at the same time points were significantly higher than that in B group (P < 0.01). The ET/NO ratio and the level of plasma endotoxin in A group were significantly lower than those in B group at each time point (P < 0.05 or 0.01). Pathological examination showed that the intestinal mucosal injury in the A group was obviously milder than that in the B group.</p><p><b>CONCLUSION</b>Oral L-arginine was shown to have the effects to ameliorate ischemia reperfusion injury of the intestine and to protect the barrier function of the intestinal mucosa. This might be related to an increase in the NO level in intestinal mucosa resulting in maintenance of a stable ET/NO ratio.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Arginine
/
Therapeutics
/
Burns
/
Reperfusion Injury
/
Endothelins
/
Rats, Sprague-Dawley
/
Therapeutic Uses
/
Disease Models, Animal
/
Feeding Methods
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Burns
Year:
2005
Type:
Article
Similar
MEDLINE
...
LILACS
LIS