Influence of pinacidil on the myocardial mitochondrial structure and the respiratory function in scalded rats / 中华烧伤杂志
Chinese Journal of Burns
;
(6): 170-172, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-303672
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the influence of pinacidil preconditioning on the protection of the structure and respiratory function of injured myocardial mitochondria in scalded rats.</p><p><b>METHODS</b>Seventy-five healthy Wistar rats, weighed 250 approximately 300 g, were randomly divided into three groups i.e. control (n = 9, with intraperitoneal injection of 50 microg/kg isotonic saline), scald (n = 33, with 30% TBSA full thickness scald) and pre-conditioning (n = 33, with same extent of scald injury after intraperitoneal injection of 50 microg/kg pinacidil) groups. Mitochondrial ultrastructure was observed by transmission electron microscope. The mitochondrial respiratory function, the MDA content and the superoxide anion level were determined with corresponding methods.</p><p><b>RESULTS</b>The degree of injury to rat myocardial mitochondria in pre-conditioning group was less intensive than that in scald group (P < 0.05 or 0.01). The respiratory control rate in pre-conditioning group was obviously higher than that in scald group (P < 0.05), and the contents of MDA and superoxide anion in pre-conditioning group were markedly lower than those in scald group (P < 0.05 or 0.01), as evidenced by their contents at 3 post scalding hours (0.60 +/- 0.09 micromol/g and 0.127 +/- 0.020) were obviously lower than those in scald group (0.83 +/- 0.07 micromol/g and 0.169 +/- 0.015) (P < 0.01).</p><p><b>CONCLUSION</b>Pinacidil preconditioning was beneficial in the protection of myocardial mitochondria in scalded rats, and it might be related to the pre-opening of potassium channel which was sensitive to mitochondrial ATP.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Burns
/
Rats, Wistar
/
Superoxides
/
Cell Respiration
/
Pinacidil
/
Therapeutic Uses
/
Disease Models, Animal
/
Drug Therapy
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Burns
Year:
2005
Type:
Article
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