The role of myeloid differentiation protein-2 in lipopolysaccharide-induced cellular activation of human endothelial cells / 中华烧伤杂志
Chinese Journal of Burns
;
(6): 97-99, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-303686
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of myeloid differentiation protein-2 (MD-2) in the human endothelial cells and its role in lipopolysaccharide (LPS)-induced NF-kappaB activation in endothelial cells.</p><p><b>METHODS</b>In vitro cultured human umbilical vein endothelial cells (HUVEC) were employed in the study. The expression of MD-2 mRNA and protein, and the effect of LPS on the expression of its mRNA and protein were assessed with RT-PCR and Western blotting. The role of MD-2 in LPS-induced NF-kappaB activation and IL-8 production were investigated with gene transfection of mutant MD-2 cDNA (0.5, 1.0, 2.0 microg), pEF-BOS vacant vector (2.0 microg) and MD-2 plasmid (2.0 microg) into HUVEC, respectively.</p><p><b>RESULTS</b>There was MD-2 mRNA and protein expression in HUVECs before LPS stimulation, and it could be obviously upregulated by LPS in time and dose-dependent manner (MD-2 protein absorbency was 25 196 +/- 1 723 without LPS stimulation, which was obviously lower than that stimulated with 0.01 mg/L LPS (58 817 +/- 3 241, P < 0.01) for 6 hours. Transfection of mutant MD-2 cDNA could remarkably inhibit LPS-induced NF-kappaB activation and IL-8 production in endothelial cells.</p><p><b>CONCLUSION</b>MD-2 might play an important role in the LPS-induced NF-kappaB activation in HUVECs.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Umbilical Veins
/
Cell Differentiation
/
Cells, Cultured
/
Lipopolysaccharides
/
Interleukin-8
/
NF-kappa B
/
Interleukin-6
/
Cell Biology
/
Endothelial Cells
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Burns
Year:
2005
Type:
Article
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