Influence of hyperoxic fluid on the down-regulated proteins of intestinal mucosa in scalded rats / 中华烧伤杂志
Chinese Journal of Burns
;
(6): 60-62, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-303692
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the influence of hyperoxic fluid on the down-regulated proteins of intestinal mucosa in scalded rats, so as to provide a theoretical basis for the clinical use of hyperoxic fluid.</p><p><b>METHODS</b>Sprague-Dawley (SD) rats with 35% TBSA full-thickness scald were randomly divided into scald control (S, n = 6,with intraperitoneal fluid infusion after scalding), hyperoxic (H, n = 6, with hyperoxic fluid infusion after scalding) groups. Six rats without scald injury served as normal group. The proteins in the intestinal mucosa were separated with the two-dimensional electrophoresis (2-DE), and were analyzed with ImageMaster 2D Elite. The influence of hyperoxic fluid on the down-regulated proteins of intestinal mucosa in scalded rats was studied with bio-spectrum, protein bank and document analysis.</p><p><b>RESULTS</b>(1) Among the 34 down-regulated protein spots in S group, 9 of them definitely exhibited up-regulation compared with those in H group. (2) The expression of mitochondrial aconitase, alpha-propionyl-CoA carboxylase, short chain of hydroxyacyl-Coenzyme A dehydrogenase, transcription factor EB (estradiol benzoate), triosephosphate isomerase 1, T cell receptor V delta 6, and dynein-like protein-5 in H group were significantly up-regulated.</p><p><b>CONCLUSION</b>The hyperoxic fluid could up-regulate the down-regulated proteins in rat intestinal mucosa at early postburn stage, so that the barrier function of intestinal mucosa of rats with severe burns could be partially recovered.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxygen
/
Therapeutics
/
Burns
/
Proteins
/
Rats, Sprague-Dawley
/
Disease Models, Animal
/
Intestinal Mucosa
/
Metabolism
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Burns
Year:
2005
Type:
Article
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