Study on the anti-maturation features of immature dendritic cells induced by low dose of granulocyte macrophage colony stimulating factor / 中华烧伤杂志
Chinese Journal of Burns
;
(6): 327-329, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-303724
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the influence of maturative agents, including lipopolysaccharide (LPS), tumor necrosis factor alpha (TNF-alpha) and interferon gamma(IFN-gamma) on the maturation of immature dendritic cells originated from murine bone marrow induced by low dose of granulocyte macrophage colony stimulating factor (rm GM-CSF).</p><p><b>METHODS</b>Dendritic cells from murine bone marrow progenitors were cultured in low and high doses of GM-CSF for 6 days, and then the suspending cells were harvested for the experiment. After 3 days of co-culture of the obtained DC with low dose rmGM-CSF (GM(low)DC) with LPS, TNF-alpha and IFN-gamma, the stimulatory capacity of inducing proliferation of non-sensitized splenocytes of GM(low)DC in mixed lymphocyte reaction (MLR) was observed and compared with that of GM(high)DC.</p><p><b>RESULTS</b>GM(low)DC could not activate the non-sensitized splenocytes or induce it into proliferation after 3 days of co-incubation with LPS, TNF-alpha, IFN-gamma, with the stimulation index (SI) lower than 2. Whereas GM(high)DC could strongly activate naive splenocytes (SI = 4.71).</p><p><b>CONCLUSION</b>GM(low)DC was resistant to maturation and insensitive to the stimulation by LPS, TNF-alpha or IFN-gamma.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Spleen
/
Dendritic Cells
/
Bone Marrow Cells
/
Lymphocyte Activation
/
T-Lymphocytes
/
Cell Differentiation
/
Cells, Cultured
/
Lipopolysaccharides
/
Granulocyte-Macrophage Colony-Stimulating Factor
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Burns
Year:
2004
Type:
Article
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